Title |
The listeriolysin O gene: A chromosomal locus crucial for the virulence of Listeria monocytogenes
|
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Published in |
Infection, March 1988
|
DOI | 10.1007/bf01639740 |
Pubmed ID | |
Authors |
P. Cossart |
Abstract |
In culture supernatants of a Tn 1545-induced non-hemolytic mutant of Listeria monocytogenes, by immunoblotting with an anti-serum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52,000D (the secreted listeriolysin O is 60,000D). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame. The homologies detected between this ORF, streptolysin O and pneumolysin demonstrate the the transposon had indeed inserted in the listeriolysin O gene. As the non-hemolytic mutant was non-virulent, our work demonstrated that a genetic determinant essential for virulence is the listeriolysin O gene or its adjacent region. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 14% |
Student > Ph. D. Student | 4 | 14% |
Researcher | 4 | 14% |
Student > Master | 4 | 14% |
Student > Postgraduate | 4 | 14% |
Other | 3 | 11% |
Unknown | 5 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 32% |
Biochemistry, Genetics and Molecular Biology | 3 | 11% |
Immunology and Microbiology | 3 | 11% |
Veterinary Science and Veterinary Medicine | 2 | 7% |
Engineering | 2 | 7% |
Other | 3 | 11% |
Unknown | 6 | 21% |