Title |
Perinatal Asphyxia and Brain Development: Mitochondrial Damage Without Anatomical or Cellular Losses
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Published in |
Molecular Neurobiology, March 2018
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DOI | 10.1007/s12035-018-1019-7 |
Pubmed ID | |
Authors |
Jean Pierre Mendes Lima, Danielle Rayêe, Thaia Silva-Rodrigues, Paula Ribeiro Paes Pereira, Ana Paula Miranda Mendonca, Clara Rodrigues-Ferreira, Diego Szczupak, Anna Fonseca, Marcus F. Oliveira, Flavia Regina Souza Lima, Roberto Lent, Antonio Galina, Daniela Uziel |
Abstract |
Perinatal asphyxia remains a significant cause of neonatal mortality and is associated with long-term neurodegenerative disorders. In the present study, we evaluated cellular and subcellular damages to brain development in a model of mild perinatal asphyxia. Survival rate in the experimental group was 67%. One hour after the insult, intraperitoneally injected Evans blue could be detected in the fetuses' brains, indicating disruption of the blood-brain barrier. Although brain mass and absolute cell numbers (neurons and non-neurons) were not reduced after perinatal asphyxia immediately and in late brain development, subcellular alterations were detected. Cortical oxygen consumption increased immediately after asphyxia, and remained high up to 7 days, returning to normal levels after 14 days. We observed an increased resistance to mitochondrial membrane permeability transition, and calcium buffering capacity in asphyxiated animals from birth to 14 days after the insult. In contrast to ex vivo data, mitochondrial oxygen consumption in primary cell cultures of neurons and astrocytes was not altered after 1% hypoxia. Taken together, our results demonstrate that although newborns were viable and apparently healthy, brain development is subcellularly altered by perinatal asphyxia. Our findings place the neonate brain mitochondria as a potential target for therapeutic protective interventions. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 39 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 7 | 18% |
Researcher | 5 | 13% |
Student > Doctoral Student | 4 | 10% |
Student > Ph. D. Student | 3 | 8% |
Student > Master | 3 | 8% |
Other | 5 | 13% |
Unknown | 12 | 31% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 8 | 21% |
Neuroscience | 5 | 13% |
Biochemistry, Genetics and Molecular Biology | 4 | 10% |
Psychology | 3 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
Other | 5 | 13% |
Unknown | 12 | 31% |