Title |
Identification of the distinct promoters for the two transcripts of apoptosis related protein 3 and their transcriptional regulation by NFAT and NFκB
|
---|---|
Published in |
Molecular and Cellular Biochemistry, March 2007
|
DOI | 10.1007/s11010-007-9440-7 |
Pubmed ID | |
Authors |
Guodong Yang, Fang Yu, Haiyan Fu, Fan Lu, Bo Huang, Liyuan Bai, Zhongliang Zhao, Libo Yao, Zifan Lu |
Abstract |
APR3 (apoptosis related protein 3) is a novel gene highly conserved across species. Analysis of the data about APR3 available at GEO profiles revealed consistent and significant changes of APR3 expression level in certain developmental and inflammatory processes. Based on the search and analysis of all the submitted mRNA sequence, we postulated that the two transcripts may arise from separate promoter activities rather than previously assumed alternative splicing. Through reporter assay and PCR data, we identified the distinct promoters for the two transcripts of APR3. Furthermore, exogenous expression of a constitutively active mutant of transcription factor NFAT was able to enhance both the promoter activities of APR3. Sequential deletion of the promoter from the 5' side and mutation of the promoter suggested the functional NFAT binding sites might localize between -96 bp and -47 bp. In contrast, exogenous expression of a constitutively active mutant of the transcription factor NFkB inhibited APR3 transcription. Our data suggested that APR3 might be functionally important in certain processes under which NFAT and/or NFkappaB are/is activated. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 22% |
Other | 1 | 11% |
Professor | 1 | 11% |
Student > Master | 1 | 11% |
Researcher | 1 | 11% |
Other | 1 | 11% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 33% |
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Immunology and Microbiology | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Unknown | 2 | 22% |