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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Detection of protein-protein interactions in live cells and animals with split firefly luciferase protein fragment complementation.
|
|---|---|
| Chapter number | 23 |
| Book title |
Genomics Protocols
|
| Published in |
Methods in molecular biology, January 2008
|
| DOI | 10.1007/978-1-59745-188-8_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-871-3, 978-1-59745-188-8
|
| Authors |
Victor Villalobos, Snehal Naik, David Piwnica-Worms, Villalobos, Victor, Naik, Snehal, Piwnica-Worms, David |
| Abstract |
Protein fragment complementation has emerged as a powerful tool for measuring protein-protein interactions in the context of live cells. The adaptation of this strategy for use with firefly luciferase now allows for the non-invasive, quantitative, real-time readout of protein interactions in lysates, live cells, and whole animals. Bioluminescence provides a robust imaging modality due to its extremely low background signal and large dynamic range. The split luciferase fusion constructs described here are inducible by addition of ligands, small molecules or drugs, in this example, rapamycin, and have been shown to work in vivo. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 4% |
| United States | 2 | 4% |
| Malaysia | 1 | 2% |
| United Kingdom | 1 | 2% |
| Unknown | 50 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 25% |
| Student > Ph. D. Student | 13 | 23% |
| Other | 7 | 13% |
| Student > Master | 4 | 7% |
| Student > Bachelor | 3 | 5% |
| Other | 11 | 20% |
| Unknown | 4 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 54% |
| Biochemistry, Genetics and Molecular Biology | 6 | 11% |
| Medicine and Dentistry | 5 | 9% |
| Unspecified | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 8 | 14% |
| Unknown | 3 | 5% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2013.
All research outputs
#18,351,676
of 22,727,570 outputs
Outputs from Methods in molecular biology
#7,858
of 13,085 outputs
Outputs of similar age
#146,655
of 155,968 outputs
Outputs of similar age from Methods in molecular biology
#72
of 87 outputs
Altmetric has tracked 22,727,570 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,085 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 155,968 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 87 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.