You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Growing Poxviruses and Determining Virus Titer
|
---|---|
Chapter number | -1592597789 |
Book title |
Vaccinia Virus and Poxvirology
|
Published in |
Methods in molecular biology, January 2004
|
DOI | 10.1385/1-59259-789-0:101 |
Pubmed ID | |
Book ISBNs |
978-1-58829-229-2, 978-1-59259-789-5
|
Authors |
Girish J. Kotwal, Melissa-Rose Abrahams |
Abstract |
Poxviruses are cell-associated viruses that can be grown in adherent- or suspension-cell cultures or chorioallantoic membranes of embryonated hen's eggs. The main principle of isolating the virus is to mechanically lyse infected cells. The virus can then be semipurified by centrifugation through a sucrose cushion. Further purification can be achieved with a sucrose gradient. This chapter describes methods for the preparation of large-scale growth and purification of the virus. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Peru | 1 | 5% |
Unknown | 20 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 24% |
Student > Master | 4 | 19% |
Student > Ph. D. Student | 3 | 14% |
Student > Bachelor | 2 | 10% |
Professor > Associate Professor | 2 | 10% |
Other | 2 | 10% |
Unknown | 3 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 38% |
Immunology and Microbiology | 3 | 14% |
Biochemistry, Genetics and Molecular Biology | 2 | 10% |
Veterinary Science and Veterinary Medicine | 2 | 10% |
Medicine and Dentistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 19% |