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Biological Aging

Overview of attention for book
Cover of 'Biological Aging'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Cell senescence culturing methods.
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    Chapter 2 Digital Image Analysis of Cells Stained with the Senescence-Associated β-Galactosidase Assay
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    Chapter 3 Analysis of biomarkers of caloric restriction in aging cells.
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    Chapter 4 Cell Sorting of Young and Senescent Cells
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    Chapter 5 Biological Aging
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    Chapter 6 Methods for Creating Mutations in C. elegans That Extend Lifespan
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    Chapter 7 Aging Studies in Drosophila Melanogaster
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    Chapter 8 The Use of Calorie Restriction Mimetics to Study Aging
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    Chapter 9 Using Somatic-Cell Nuclear Transfer to Study Aging
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    Chapter 10 Induction of Cellular Senescence by Oncogenic RAS
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    Chapter 11 Methods of Cellular Senescence Induction Using Oxidative Stress
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    Chapter 12 Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice
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    Chapter 13 Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors
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    Chapter 14 Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration
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    Chapter 15 Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging
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    Chapter 16 Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequencing.
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    Chapter 17 Gel Electrophoresis-Based Proteomics of Senescent Tissues
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    Chapter 18 Single-Cell Semiconductor Sequencing
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    Chapter 19 Application of DNA Microarray Technology to Gerontological Studies
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    Chapter 20 Epigenetic biomarker to determine replicative senescence of cultured cells.
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    Chapter 21 Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging
Attention for Chapter 20: Epigenetic biomarker to determine replicative senescence of cultured cells.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

blogs
1 blog

Citations

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4 Dimensions

Readers on

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15 Mendeley
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Chapter title
Epigenetic biomarker to determine replicative senescence of cultured cells.
Chapter number 20
Book title
Biological Aging
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-556-9_20
Pubmed ID
Book ISBNs
978-1-62703-555-2, 978-1-62703-556-9
Authors

Carmen M Koch, Wolfgang Wagner, Carmen M. Koch

Abstract

Somatic cells change continuously during culture expansion-long-term culture evokes increasing cell size, declining differentiation potential, and ultimate cell cycle arrest upon senescence. These changes are of particular relevance for cellular therapy which necessitates standardized products and reliable quality control. Recently, replicative senescence has been shown to be associated with highly reproducible epigenetic modifications. Here, we describe a simple method to track the state of senescence in mesenchymal stromal cells (MSCs) or fibroblasts by monitoring continuous DNA methylation (DNAm) changes at specific sites in the genome. Six CpG sites have been identified which reveal either linear hypermethylation or hypomethylation with respect to the number of cumulative population doublings (cPDs). Conversely, the DNAm level at these CpG sites can be analyzed-for example, by pyrosequencing of bisulfite-converted DNA-and then used for linear regression models to predict cPDs. Our method provides an epigenetic biomarker to determine the state of senescence in cell preparations.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 7%
Sweden 1 7%
France 1 7%
Unknown 12 80%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Researcher 3 20%
Professor 2 13%
Student > Master 2 13%
Other 1 7%
Other 2 13%
Unknown 1 7%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 40%
Medicine and Dentistry 3 20%
Agricultural and Biological Sciences 2 13%
Psychology 1 7%
Neuroscience 1 7%
Other 1 7%
Unknown 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 November 2013.
All research outputs
#517,025
of 3,627,074 outputs
Outputs from Methods in molecular biology
#91
of 2,355 outputs
Outputs of similar age
#17,777
of 108,322 outputs
Outputs of similar age from Methods in molecular biology
#11
of 330 outputs
Altmetric has tracked 3,627,074 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,355 research outputs from this source. They receive a mean Attention Score of 1.4. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 108,322 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 330 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.