| Chapter title |
Isolation and Purification of Exosomes in Urine
|
|---|---|
| Chapter number | 6 |
| Book title |
The Urinary Proteome
|
| Published in |
Methods in molecular biology, January 2010
|
| DOI | 10.1007/978-1-60761-711-2_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-60761-710-5, 978-1-60761-711-2
|
| Authors |
Patricia A. Gonzales, Hua Zhou, Trairak Pisitkun, Nam Sun Wang, Robert A. Star, Mark A. Knepper, Peter S. T. Yuen |
| Abstract |
Exosomes represent an important and readily isolated subset of the urinary proteome that has the potential to shed much insight on the health status of the kidney. Each segment of the nephron sheds exosomes into the urine. Exosomes are rich in potential biomarkers, especially membrane proteins such as transporters and receptors that may be up- or downregulated during disease states. Two differential centrifugation methods are available for simple purification of exosomes: one uses ultracentrifugation, and the other uses a nanomembrane concentrator. Validation methods include western blots of pan-exosome markers and segment-specific exosome markers, and negative staining electron microscopy. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Korea, Republic of | 1 | <1% |
| Brazil | 1 | <1% |
| South Africa | 1 | <1% |
| Russia | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 127 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 18% |
| Researcher | 22 | 17% |
| Student > Master | 15 | 11% |
| Student > Doctoral Student | 10 | 8% |
| Student > Bachelor | 8 | 6% |
| Other | 25 | 19% |
| Unknown | 28 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 25% |
| Biochemistry, Genetics and Molecular Biology | 28 | 21% |
| Medicine and Dentistry | 19 | 14% |
| Engineering | 5 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
| Other | 10 | 8% |
| Unknown | 33 | 25% |