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Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac

Overview of attention for article published in Insect Biochemistry & Molecular Biology, April 2015
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2 tweeters

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Title
Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac
Published in
Insect Biochemistry & Molecular Biology, April 2015
DOI 10.1016/j.ibmb.2015.01.014
Pubmed ID
Authors

Zuwen Chen, Fei He, Yutao Xiao, Chenxi Liu, Jianghuai Li, Yongbo Yang, Hui Ai, Jianxin Peng, Huazhu Hong, Kaiyu Liu

Abstract

Although many insect cell lines derived from various tissues are available, it is unclear whether endogenous receptors of Bacillus thuringiensis (Bt) crystal toxins are expressed in these cell lines. In the present study, we demonstrated that the ovaries-derived Spodoptera litura Sl-HP cell line was susceptible to activated Cry1Ac although larvae of S. litura are not susceptible to the toxin. Assays of the transcriptome revealed that thirteen ATP-binding cassette transporter genes (ABC) were expressed at different levels in this cell line. Of these, the SlABCC3 shared 52-55% amino acid sequence identity with the known Bt toxin receptor ABCC2. RNAi-mediated knockdown targeting SlABCC3 significantly decreased the susceptibility of Sl-HP cells to activated Cry1Ac. Over-expression of the gene strongly increased the susceptibility of Trichoplusia ni Hi5 cells to the toxin. Not only was SlABCC3 comparable to the heterologously expressed Helicoverpa armigera Hacadherin on the receptor-mediated cytotoxicity of activated Cry1Ac to Hi5 cells, but also SlABCC3 and Hacadherin had a strong synergistic effect on cytotoxicity of activated Cry1Ac. These results suggested that Bt toxin receptors-expressing insect cell lines can be used as an alternative model for evaluating cytotoxicity of Bt toxins and studying their mechanisms of action.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 28 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 28%
Student > Ph. D. Student 7 24%
Student > Master 6 21%
Professor 2 7%
Student > Bachelor 2 7%
Other 4 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 62%
Biochemistry, Genetics and Molecular Biology 5 17%
Unspecified 2 7%
Nursing and Health Professions 1 3%
Environmental Science 1 3%
Other 2 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2016.
All research outputs
#9,810,887
of 12,282,412 outputs
Outputs from Insect Biochemistry & Molecular Biology
#682
of 874 outputs
Outputs of similar age
#185,850
of 271,091 outputs
Outputs of similar age from Insect Biochemistry & Molecular Biology
#21
of 29 outputs
Altmetric has tracked 12,282,412 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 874 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,091 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.