| Chapter title |
QTL Mapping by Pooled-Segregant Whole-Genome Sequencing in Yeast.
|
|---|---|
| Chapter number | 15 |
| Book title |
Yeast Metabolic Engineering
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-0563-8_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0562-1, 978-1-4939-0563-8
|
| Authors |
Thiago M Pais, María R Foulquié-Moreno, Johan M Thevelein, Thiago M. Pais, María R. Foulquié-Moreno, Johan M. Thevelein |
| Abstract |
Quantitative trait locus (QTL) mapping by pooled-segregant whole-genome sequencing in yeast is a robust methodology for the simultaneous identification of superior genes involved in polygenic traits (e.g., high ethanol tolerance). By crossing two haploid strains with opposite phenotypes, being one of interest, the resulting diploid is sporulated, the meiotic segregants phenotyped, and a pool of selected segregants with the phenotype of interest assembled. The genotyping by pooled-segregant sequencing constitutes a fast and reliable methodology to map all QTL defining the trait of interest. The QTLs can be further analyzed by reciprocal hemizygosity analysis to identify the causative superior alleles that can subsequently be used for yeast strain improvement by targeted genetic engineering. |
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