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High Throughput Protein Expression and Purification

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Cover of 'High Throughput Protein Expression and Purification'

Table of Contents

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    Book Overview
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    Chapter 1 High-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production
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    Chapter 2 Designing Experiments for High-Throughput Protein Expression
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    Chapter 3 Gateway cloning for protein expression.
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    Chapter 4 Flexi Vector Cloning
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    Chapter 5 The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning
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    Chapter 6 The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis.
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    Chapter 7 A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
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    Chapter 8 “System 48” High-Throughput Cloning and Protein Expression Analysis
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    Chapter 9 Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on MagneHis Ni 2 +-Particles
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    Chapter 10 E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis
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    Chapter 11 Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.
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    Chapter 12 PHB-Intein-Mediated Protein Purification Strategy
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    Chapter 13 High-throughput biotinylation of proteins.
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    Chapter 14 High-Throughput Insect Cell Protein Expression Applications
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    Chapter 15 High-Throughput Protein Expression Using Cell-Free System
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    Chapter 16 The Production of Glycoproteins by Transient Expression in Mammalian Cells
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    Chapter 17 High-Throughput Expression and Detergent Screening of Integral Membrane Proteins
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    Chapter 18 Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform
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    Chapter 19 Expression and purification of soluble His(6)-tagged TEV protease.
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    Chapter 20 High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation
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