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Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, February 2014
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Title
Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni
Published in
Memórias do Instituto Oswaldo Cruz, February 2014
DOI 10.1590/0074-0276130107
Pubmed ID
Authors

Roberta Verciano Pereira, Helaine Graziele Santos Vieira, Victor Fernandes de Oliveira, Matheus de Souza Gomes, Liana Konovaloff Jannotti Passos, William de Castro Borges, Renata Guerra-Sá

Abstract

Several genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and shown to play an important role in Ub-dependent processes, little is known about their role in S. mansoni . In this study, we analysed the Ub carboxyl-terminal hydrolase (UCHs) proteins found in the database of the parasite's genome. An in silico ana- lysis (GeneDB and MEROPS) identified three different UCH family members in the genome, Sm UCH-L3, Sm UCH-L5 and Sm BAP-1 and a phylogenetic analysis confirmed the evolutionary conservation of the proteins. We performed quantitative reverse transcription-polymerase chain reaction and observed a differential expression profile for all of the investigated transcripts between the cercariae and adult worm stages. These results were corroborated by low rates of Z-Arg-Leu-Arg-Gly-Gly-AMC hydrolysis in a crude extract obtained from cercariae in parallel with high Ub conjugate levels in the same extracts. We suggest that the accumulation of ubiquitinated proteins in the cercaria and early schistosomulum stages is related to a decrease in 26S proteasome activity. Taken together, our data suggest that UCH family members contribute to regulating the activity of the Ub-proteasome system during the life cycle of this parasite.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 13%
Unknown 14 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 38%
Student > Master 3 19%
Researcher 3 19%
Student > Bachelor 1 6%
Unspecified 1 6%
Other 1 6%
Unknown 1 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 38%
Biochemistry, Genetics and Molecular Biology 3 19%
Chemistry 2 13%
Medicine and Dentistry 2 13%
Unspecified 1 6%
Other 1 6%
Unknown 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2015.
All research outputs
#11,127,489
of 12,511,869 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#753
of 862 outputs
Outputs of similar age
#223,696
of 273,549 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#7
of 9 outputs
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So far Altmetric has tracked 862 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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