Chapter title |
Cloning of a putative GABAA receptor from cyclodiene-resistant Drosophila: a case study in the use of insecticide-resistant mutants to isolate neuroreceptors.
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Chapter number | 9 |
Book title |
Comparative Molecular Neurobiology
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Published in |
EXS, January 1993
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DOI | 10.1007/978-3-0348-7265-2_9 |
Pubmed ID | |
Book ISBNs |
978-3-03-487267-6, 978-3-03-487265-2
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Authors |
ffrench-Constant, R H, R. H. ffrench-Constant, ffrench-Constant, R. H. |
Abstract |
This chapter uses the isolation and cloning of cyclodiene resistance from Drosophila melanogaster to illustrate how mutants resistant to a toxicant can be used to study neuroreceptors. Isolation of mutants from the field, mapping of the single gene responsible and its subsequent cloning are described. As confirmation of gene cloning a susceptible allele of the gene has been used to genetically transform resistant individuals to susceptibility. The gene product appears to code for a subunit of a receptor highly similar to vertebrate GABAA receptor/chloride ion channels, and functional expression studies are described which will elucidate its pharmacology. Cyclodiene resistance is extremely widespread, occurring in both invertebrates and vertebrates. Thus examination of resistance-associated mutations in this receptor in a range of species will enhance our understanding of both the binding sites of toxic ligands and the genetic basis of pesticide resistance. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 50% |
Student > Postgraduate | 2 | 20% |
Professor | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Other | 1 | 10% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 40% |
Neuroscience | 2 | 20% |
Medicine and Dentistry | 2 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 10% |
Environmental Science | 1 | 10% |
Other | 0 | 0% |