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Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds

Overview of attention for article published in BMC Complementary and Alternative Medicine, April 2018
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Title
Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds
Published in
BMC Complementary and Alternative Medicine, April 2018
DOI 10.1186/s12906-018-2176-y
Pubmed ID
Authors

Raid Al Akeel, Ayesha Mateen, Khalid K. Alharbi, Abdullah A. Alyousef, Hazem M. Al-Mandeel, Rabbani Syed

Abstract

Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action. Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs. Antimicrobial peptides were extracted from seeds using a sodium phosphate citrate (pH 7.2) - CTAB cradle (pH 6.0). The highest protein concentration was seen with elute fractions 1 and 3 (370 mg/mL) compared with elute fractions 2 and 4 (340 mg/mL). Among the bacteria utilized, E. coli was clearly the most sensitive out of selected four strains. Our results suggest that Cucumis sativus L seeds extracts have significant potentials as new antimicrobial agents.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 29%
Researcher 4 17%
Student > Ph. D. Student 2 8%
Other 1 4%
Student > Doctoral Student 1 4%
Other 3 13%
Unknown 6 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 17%
Biochemistry, Genetics and Molecular Biology 4 17%
Pharmacology, Toxicology and Pharmaceutical Science 2 8%
Chemistry 2 8%
Immunology and Microbiology 2 8%
Other 2 8%
Unknown 8 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2018.
All research outputs
#11,349,799
of 12,761,472 outputs
Outputs from BMC Complementary and Alternative Medicine
#2,143
of 2,605 outputs
Outputs of similar age
#238,096
of 273,558 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#1
of 1 outputs
Altmetric has tracked 12,761,472 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,605 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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