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Mendeley readers
| Chapter title |
Synthesis and Characterization of Aptamer-Targeted SNALPs for the Delivery of siRNA.
|
|---|---|
| Chapter number | 18 |
| Book title |
Nucleic Acid Aptamers
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3197-2_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3196-5, 978-1-4939-3197-2
|
| Authors |
Wilner, Samantha E, Levy, Matthew, Samantha E. Wilner, Matthew Levy, Wilner, Samantha E. |
| Abstract |
Aptamers selected against cell surface receptors represent a unique set of ligands that can be used to target nanoparticles and other therapeutics to specific cell types. Here, we describe a method for using aptamers to deliver stable nucleic acid lipid particles (SNALPs) encapsulating small interfering RNA (siRNA) to cells in vitro. Using this method, we have demonstrated the ability of aptamer-conjugated SNALPs to achieve target-specific delivery and siRNA-mediated knockdown of a gene of interest. We also describe methods to characterize SNALP size, siRNA encapsulation efficiency, and aptamer conjugation efficiency. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 35% |
| Student > Master | 5 | 25% |
| Student > Ph. D. Student | 2 | 10% |
| Student > Bachelor | 1 | 5% |
| Unknown | 5 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 20% |
| Agricultural and Biological Sciences | 3 | 15% |
| Chemistry | 3 | 15% |
| Immunology and Microbiology | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 6 | 30% |