Chapter title |
A newly identified MHV-A59 ORF1a polypeptide p65 is temperature sensitive in two RNA negative mutants.
|
---|---|
Chapter number | 35 |
Book title |
Coronaviruses
|
Published in |
Advances in experimental medicine and biology, January 1993
|
DOI | 10.1007/978-1-4615-2996-5_35 |
Pubmed ID | |
Book ISBNs |
978-1-4613-6305-7, 978-1-4615-2996-5
|
Authors |
Hughes, S A, Denison, M R, Bonilla, P, Leibowitz, J L, Baric, R S, Weiss, S R, Scott A. Hughes, Mark R. Denison, Pedro Bonilla, Julian L. Leibowitz, Ralph S. Baric, Susan R. Weiss, Hughes, Scott A., Denison, Mark R., Bonilla, Pedro, Leibowitz, Julian L., Baric, Ralph S., Weiss, Susan R. |
Abstract |
Polypeptide products of MHV-A59 gene 1 have been identified in infected DBT cells and in the products of in vitro translations of genome RNA. In this paper we report the identification in infected cell lysates of a 65-kDa polypeptide (p65) encoded in ORF 1a. Studies on the kinetics of appearance and processing of p65 show that p65 is detectable after p28 but before the appearance of p290, p240 and p50. No homologue of the p65 polypeptide identified in infected cell lysates was immunoprecipitated from in vitro translations of genomic RNA, providing further evidence that in vitro processing of polypeptides encoded in ORF 1a of gene 1 differs from that which occurs late in infection of DBT cells. Although the function of p65 is unknown, two MHV-A59 ts mutants isolated and characterized by Baric et al. (3,4) do not produce detectable levels of p65 at the non-permissive temperature indicating that p65 may play an important role in the virus life cycle. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 31% |
Professor > Associate Professor | 3 | 23% |
Other | 1 | 8% |
Professor | 1 | 8% |
Student > Bachelor | 1 | 8% |
Other | 2 | 15% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 23% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Engineering | 2 | 15% |
Psychology | 1 | 8% |
Immunology and Microbiology | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |