| Chapter title |
Isolation, Propagation, and Analysis of Biological Nanoparticles.
|
|---|---|
| Chapter number | 20 |
| Book title |
Nanoproteomics
|
| Published in |
Methods in molecular biology, January 2011
|
| DOI | 10.1007/978-1-61779-319-6_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-318-9, 978-1-61779-319-6
|
| Authors |
Michael P. Linnes, Farooq A. Shiekh, Larry W. Hunter, Virginia M. Miller, John C. Lieske, Linnes, Michael P., Shiekh, Farooq A., Hunter, Larry W., Miller, Virginia M., Lieske, John C. |
| Abstract |
Calcifying biologic nanoparticles (NPs) have been implicated as nucleation points for a number of -pathologic events that include vascular calcification and the formation of kidney stones. In order to study these potential relationships, reproducible isolation of well-characterized biologic NPs is a necessity. Our group has isolated and propagated calcifying NPs from several human tissues and renal stones. Specific proteins that could nucleate a calcium phosphate shell under physiologic conditions have been identified as part of their structure, including elongation factor Tu (EF-Tu) and fetuin-A. Visualization, using advanced transmission electron microscopy (TEM), immunofluorescence microscopy, and nuclear and antibody staining in conjunction with flow cytometry, can further elucidate NPs composition and their role in pathology. In order to allow uniform investigation by others, the isolation, culture, and handling procedures for biologic NPs from human calcified vascular tissue and kidney stones are reported in detail. |
X Demographics
Geographical breakdown
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|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
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| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 17% |
| Australia | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 1 | 17% |
| Professor | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Student > Master | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
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|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 33% |
| Agricultural and Biological Sciences | 2 | 33% |
| Medicine and Dentistry | 1 | 17% |
| Unknown | 1 | 17% |