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Staphylococcus Epidermidis

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Cover of 'Staphylococcus Epidermidis'

Table of Contents

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    Book Overview
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    Chapter 1 Clinical Characteristics of Infections in Humans Due to Staphylococcus epidermidis
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    Chapter 2 Staphylococcus epidermidis Pathogenesis
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    Chapter 3 Identification of Staphylococcus epidermidis in the Clinical Microbiology Laboratory by Molecular Methods
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    Chapter 4 Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis
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    Chapter 5 Multilocus Sequence Typing of Staphylococcus epidermidis
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    Chapter 6 Growth and Preparation of Staphylococcus epidermidis for NMR Metabolomic Analysis
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    Chapter 7 The Isolation and Analysis of Phenol-Soluble Modulins of Staphylococcus epidermidis
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    Chapter 8 Genetic Manipulation of Staphylococci
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    Chapter 9 Isolation of Chromosomal and Plasmid DNA from Staphylococcus epidermidis
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    Chapter 10 Isolation of Staphylococcus sp. RNA
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    Chapter 11 Use of Electroporation and Conjugative Mobilization for Genetic Manipulation of Staphylococcus epidermidis
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    Chapter 12 Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis Strain 1457.
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    Chapter 13 Examination of Staphylococcus epidermidis Biofilms Using Flow-Cell Technology
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    Chapter 14 Rapid Quantitative and Qualitative Analysis of Biofilm Production by Staphylococcus epidermidis Under Static Growth Conditions
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    Chapter 15 Bacteriophage Transduction in Staphylococcus epidermidis
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    Chapter 16 Mouse Model of Post-arthroplasty Staphylococcus epidermidis Joint Infection
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    Chapter 17 A Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection
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    Chapter 18 Generation of a Central Nervous System Catheter-Associated Infection in Mice with Staphylococcus epidermidis
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    Chapter 19 Rat Jugular Catheter Model of Biofilm-Mediated Infection
Attention for Chapter 12: Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis Strain 1457.
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Chapter title
Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis Strain 1457.
Chapter number 12
Book title
Staphylococcus Epidermidis
Published in
Methods in molecular biology, November 2013
DOI 10.1007/978-1-62703-736-5_12
Pubmed ID
Book ISBNs
978-1-62703-735-8, 978-1-62703-736-5
Authors

Widhelm TJ, Yajjala VK, Endres JL, Fey PD, Bayles KW, Todd J. Widhelm, Vijay Kumar Yajjala, Jennifer L. Endres, Paul D. Fey, Kenneth W. Bayles, Widhelm, Todd J., Yajjala, Vijay Kumar, Endres, Jennifer L., Fey, Paul D., Bayles, Kenneth W.

Abstract

Transposon mutant libraries are valuable resources to investigators studying bacterial species, including Staphylococcus epidermidis, which are difficult to genetically manipulate. Although sequence-defined transposon mutant libraries have been constructed in Staphylococcus aureus, no such library exists for S. epidermidis. Nevertheless, the study of Tn917-mediated mutations has been paramount in discovering unique aspects of S. epidermidis biology including initial adherence and accumulation during biofilm formation. Herein, we describe modifications to the methodology first described by Bae et al. to utilize the mariner-based transposon bursa aurealis to generate mutants in S. epidermidis strain 1457.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 22%
Student > Bachelor 3 17%
Professor > Associate Professor 3 17%
Student > Ph. D. Student 3 17%
Unspecified 1 6%
Other 2 11%
Unknown 2 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 33%
Agricultural and Biological Sciences 6 33%
Unspecified 1 6%
Chemistry 1 6%
Unknown 4 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2014.
All research outputs
#20,231,392
of 22,757,090 outputs
Outputs from Methods in molecular biology
#9,863
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Outputs of similar age
#184,160
of 211,441 outputs
Outputs of similar age from Methods in molecular biology
#32
of 44 outputs
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