Quantification of intracellular mitochondrial displacements in response to nanomechanical forces.
Cellular and Subcellular Nanotechnology
Methods in molecular biology, April 2013
Silberberg YR, Pelling AE, Yaron R. Silberberg, Andrew E. Pelling
Mechanical stress affects various aspects of cell behavior, including cell growth, morphology, differentiation, and genetic expression. Here, we describe a method to quantify the intracellular mechanical response to an extracellular mechanical perturbation, specifically the displacement of mitochondria. A combined fluorescent-atomic force microscope (AFM) was used to simultaneously produce well-defined nanomechanical stimulation to a living cell while optically recording the real-time displacement of fluorescently labeled mitochondria. A single-particle tracking (SPT) approach was then applied in order to quantify the two-dimensional displacement of mitochondria in response to local forces.
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