Chapter title |
Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation.
|
---|---|
Chapter number | 19 |
Book title |
Animal Models for Stem Cell Therapy
|
Published in |
Methods in molecular biology, September 2014
|
DOI | 10.1007/978-1-4939-1453-1_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1452-4, 978-1-4939-1453-1
|
Authors |
Lau AM, Tseng YH, Schulz TJ, Anne-Marie Lau, Yu-Hua Tseng, Tim J. Schulz, Lau, Anne-Marie, Tseng, Yu-Hua, Schulz, Tim J. |
Abstract |
Skeletal muscle harbors several types of cells, among which a population of progenitors committed to the adipogenic lineage has only recently been identified. Potential sources of white and brown adipocytes, the latter representing a potential target to treat obesity, are of considerable interest to the field. Fluorescence-activated cell sorting (FACS) provides an elegant strategy for prospective isolation of closely defined cell populations. Here we describe a flow cytometric method to isolate muscle-resident adipogenic progenitor cells with a default potential to undergo white adipogenesis. We further describe an approach to induce commitment to a lineage of brown-like adipocytes upon exposure to bone morphogenetic protein 7 (BMP7). |
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