| Chapter title |
Detection of Apoptosis Using Cyclic Luciferase in Living Mammals
|
|---|---|
| Chapter number | 9 |
| Book title |
Bioluminescence
|
| Published in |
Methods in molecular biology, January 2009
|
| DOI | 10.1007/978-1-60327-321-3_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-60327-320-6, 978-1-60327-321-3
|
| Authors |
Akira Kanno, Yoshio Umezawa, Takeaki Ozawa, Kanno, Akira, Umezawa, Yoshio, Ozawa, Takeaki |
| Abstract |
Programmed cell death, apoptosis, is a crucial process involved in pathogenesis and progression of diseases, which is executed by cysteine aspartyl proteases (caspases). The caspase activities in living subjects and their regulation with small chemical compounds are of great interest for screening drug candidates or pathological agents. We describe a novel genetically encoded bioluminescent indicator for real-time imaging of caspase-3 activities in living cells and animals. The indicator is composed of an engineered firefly luciferase, of which the N- and C-terminal ends are linked with a substrate sequence of caspase-3 (Asp-Glu-Val-Asp). When activated caspase-3 digests the substrate sequence, the cyclized luciferase recovers its activity. The indicator provides a general means of evaluating effects of cytotoxic compounds or novel pharmacological chemicals and apoptosis. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 24% |
| Student > Ph. D. Student | 4 | 19% |
| Other | 3 | 14% |
| Professor | 2 | 10% |
| Student > Master | 2 | 10% |
| Other | 1 | 5% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 24% |
| Agricultural and Biological Sciences | 3 | 14% |
| Chemistry | 3 | 14% |
| Medicine and Dentistry | 3 | 14% |
| Nursing and Health Professions | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 24% |