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Fluorescence Spectroscopy and Microscopy

Overview of attention for book
Cover of 'Fluorescence Spectroscopy and Microscopy'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
  3. Altmetric Badge
    Chapter 2 Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
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    Chapter 3 Quantitative Fluorescence Spectral Analysis of Protein Denaturation
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    Chapter 4 High-Pressure Fluorescence Applications
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    Chapter 5 Frequency Domain Fluorometry: Theory and Application
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    Chapter 6 Polar Plot Representation of Time-Resolved Fluorescence
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    Chapter 7 Ensemble and Single-Molecule Detected Time-Resolved FRET Methods in Studies of Protein Conformations and Dynamics
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    Chapter 8 MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
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    Chapter 9 Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins
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    Chapter 10 Global Analysis of Time-Resolved Fluorescence Data
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    Chapter 11 Nanometrology.
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    Chapter 12 Upconversion Spectrophotofluorometry
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    Chapter 13 Subpicosecond Kerr-Gate Spectrofluorometry
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    Chapter 14 Photoinduced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay
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    Chapter 15 Biosynthetic Incorporation of Tryptophan Analogs in Proteins
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    Chapter 16 Optimization of Fluorescent Proteins
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    Chapter 17 Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family
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    Chapter 18 Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope
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    Chapter 19 A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging.
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    Chapter 20 Wide-Field Fluorescence Lifetime Imaging with Multi-anode Detectors
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    Chapter 21 Global Analysis of FRET–FLIM Data in Live Plant Cells
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    Chapter 22 Time-Resolved Fluorescence Anisotropy Imaging
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    Chapter 23 Multimodal Fluorescence Imaging Spectroscopy
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    Chapter 24 Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells
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    Chapter 25 Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells.
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    Chapter 26 Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS).
  28. Altmetric Badge
    Chapter 27 Fluorescence Spectroscopy and Microscopy
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    Chapter 28 Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes
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    Chapter 29 Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane.
  31. Altmetric Badge
    Chapter 30 Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS.
  32. Altmetric Badge
    Chapter 31 Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.
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    Chapter 32 Brightness Experiments
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    Chapter 33 Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy
  35. Altmetric Badge
    Chapter 34 Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy
  36. Altmetric Badge
    Chapter 35 Single-Molecule Fluorescence of Nucleic Acids.
  37. Altmetric Badge
    Chapter 36 Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Circumventing the Diffraction Limit of Light
Attention for Chapter 25: Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells.
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Chapter title
Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells.
Chapter number 25
Book title
Fluorescence Spectroscopy and Microscopy
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-649-8_25
Pubmed ID
24108644
Book ISBNs
978-1-62703-648-1, 978-1-62703-649-8
Authors

Xiaoxiao Ma, Yong Hwee Foo, Thorsten Wohland, Ma, Xiaoxiao, Foo, Yong Hwee, Wohland, Thorsten

Abstract

Fluorescence cross-correlation spectroscopy (FCCS) is a single-molecule sensitive technique to quantitatively study interactions among fluorescently tagged biomolecules. Besides the initial implementation as dual-color FCCS (DC-FCCS), FCCS has several powerful derivatives, including single-wavelength FCCS (SW-FCCS), two-photon FCCS (TP-FCCS), and pulsed interleaved excitation FCCS (PIE-FCCS). However, to apply FCCS successfully, one needs to be familiar with procedures ranging from fluorescent labeling, instrumentation setup and alignment, sample preparation, and data analysis. Here, we describe the procedures to apply FCCS in various biological samples ranging from live cells to in vivo measurements, with the focus on DC-FCCS and SW-FCCS.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 2%
France 1 2%
Unknown 45 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 26%
Researcher 10 21%
Student > Master 5 11%
Student > Bachelor 3 6%
Student > Doctoral Student 2 4%
Other 6 13%
Unknown 9 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 23%
Biochemistry, Genetics and Molecular Biology 9 19%
Chemistry 6 13%
Physics and Astronomy 6 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 3 6%
Unknown 11 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 October 2013.
All research outputs
#18,351,676
of 22,727,570 outputs
Outputs from Methods in molecular biology
#7,859
of 13,086 outputs
Outputs of similar age
#229,288
of 305,158 outputs
Outputs of similar age from Methods in molecular biology
#293
of 594 outputs
Altmetric has tracked 22,727,570 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,086 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,158 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 594 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.

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