Chapter title |
Detection of Protein-Protein Interaction Using Bimolecular Fluorescence Complementation Assay
|
---|---|
Chapter number | 32 |
Book title |
Protein-Protein Interactions
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2425-7_32 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
Authors |
Cau D. Pham, Pham, Cau D. |
Abstract |
The bimolecular fluorescence complementation (BiFC) assay is a versatile technique for investigating protein-protein interaction (PPI) in living systems. The BiFC assay exploits the color-emitting moiety and the modular structure of fluorescent proteins to provide both temporal and spatial information of the PPI. The modular property of fluorescent proteins enables researchers to strategically partition a fluorescent protein into two nonfluorescent units, which can be independently fused to other proteins. When the fusion proteins interact with each other, the nonfluorescent fragments reconstitute to generate a fluorescence signal. PPI can then be detected by capturing the fluorescence signal with a fluorescence microscope. In this chapter, the Venus fluorescent protein is employed to demonstrate the application of the BiFC assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 21% |
Researcher | 4 | 17% |
Student > Doctoral Student | 3 | 13% |
Student > Master | 3 | 13% |
Student > Bachelor | 1 | 4% |
Other | 3 | 13% |
Unknown | 5 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 58% |
Biochemistry, Genetics and Molecular Biology | 7 | 29% |
Unknown | 3 | 13% |