Recombinant and In Vitro RNA Synthesis

Chapter 1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold

Chapter 2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold

Chapter 3 Preparation of Long Templates for RNA In Vitro Transcription by Recursive PCR

Chapter 4 General Protocols for Preparation of Plasmid DNA Template, RNA In Vitro Transcription, and RNA Purification by Denaturing PAGE

Chapter 5 Preparation of Short RNA by In Vitro Transcription

Chapter 6 Native RNA Purification by Gel Filtration Chromatography

Chapter 7 Cis -Acting Ribozymes for the Production of RNA In Vitro Transcripts with Defined 5′ and 3′ Ends

Chapter 8 Trans- Acting Antigenomic HDV Ribozyme for Production of In Vitro Transcripts with Homogenous 3′ Ends

Chapter 9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography and DNAzyme Methods

Chapter 10 Preparation of λN-GST Fusion Protein for Affinity Immobilization of RNA

Chapter 11 Affinity Purification of RNA Using an ARiBo Tag

Chapter 12 Plasmid Template Design and In Vitro Transcription of Short RNAs Within a “Structure Cassette” for Structure Probing Experiments

Chapter 14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis

Chapter 15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA

Chapter 16 Se-Derivatized RNAs for X-ray Crystallography

Chapter 17 Biosynthetic Preparation of 13 C/ 15 N-Labeled rNTPs for High-Resolution NMR Studies of RNAs

Chapter 18 Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC

Chapter 19 Splint ligation of RNA with t4 DNA ligase.