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miRNA Maturation

Overview of attention for book
Cover of 'miRNA Maturation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Pathway of miRNA Maturation
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    Chapter 2 MicroRNA Maturation and Human Disease
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    Chapter 3 Approaches to the Modulation of miRNA Maturation
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    Chapter 4 Expression Profiling of Components of the miRNA Maturation Machinery
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    Chapter 5 Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
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    Chapter 6 In Vivo Processing Assay Based on a Dual-Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity
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    Chapter 7 Assaying Dicer-Mediated miRNA Maturation by Means of Fluorescent Substrates
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    Chapter 8 A fluorescence correlation spectroscopy-based enzyme assay for human dicer.
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    Chapter 9 Detection of microRNA Maturation Using Unmodified pre-microRNA and Branched Rolling Circle Amplification
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    Chapter 10 Quantitative RT-PCR Specific for Precursor and Mature miRNAs
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    Chapter 11 Cellular MicroRNA Sensors Based on Luciferase Reporters
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    Chapter 12 Identification of Inhibitors of MicroRNA Function from Small Molecule Screens
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    Chapter 13 Inhibition of miRNA Maturation by Peptide Nucleic Acids.
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    Chapter 14 Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs
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    Chapter 15 Lentiviral Overexpression of miRNAs
Attention for Chapter 8: A fluorescence correlation spectroscopy-based enzyme assay for human dicer.
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Chapter title
A fluorescence correlation spectroscopy-based enzyme assay for human dicer.
Chapter number 8
Book title
miRNA Maturation
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-703-7_8
Pubmed ID
24166306
Book ISBNs
978-1-62703-702-0, 978-1-62703-703-7
Authors

Eileen Magbanua, Ulrich Hahn, Magbanua, Eileen, Hahn, Ulrich

Abstract

We used fluorescence correlation spectroscopy (FCS) to establish an in vitro assay to investigate RNase activity of human Dicer (Werner et al., Biol Chem 393(3):187-193). FCS allows investigating the interactions of different particles due to their differing diffusion mobility, provided that one of the interacting partners exhibit a fluorescence label. In our case we used a fluorophore-labeled double-stranded RNA (dsRNA) as substrate to monitor Dicer activity. The dsRNA was cleaved by the enzyme resulting in a five-nucleotide-short single-stranded RNA (ssRNA) fragment carrying the fluorophore, which could be distinguished from the substrate and unlabeled second product by FCS. Furthermore, we refer to additional (control) experiments to confirm obtained data.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 10%
Unknown 9 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Student > Ph. D. Student 3 30%
Other 1 10%
Student > Bachelor 1 10%
Student > Master 1 10%
Other 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Biochemistry, Genetics and Molecular Biology 3 30%
Nursing and Health Professions 1 10%
Computer Science 1 10%
Social Sciences 1 10%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2013.
All research outputs
#18,353,475
of 22,729,647 outputs
Outputs from Methods in molecular biology
#7,859
of 13,086 outputs
Outputs of similar age
#229,288
of 305,158 outputs
Outputs of similar age from Methods in molecular biology
#293
of 594 outputs
Altmetric has tracked 22,729,647 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,086 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,158 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 594 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.

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