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Plant Cell Division

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Cover of 'Plant Cell Division'

Table of Contents

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    Book Overview
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    Chapter 1 Synchronization of Green Algae by Light and Dark Regimes for Cell Cycle and Cell Division Studies
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    Chapter 2 Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells
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    Chapter 3 Identifying Gene Regulatory Networks in Arabidopsis by In Silico Prediction, Yeast-1-Hybrid, and Inducible Gene Profiling Assays.
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    Chapter 4 Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis
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    Chapter 5 Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors
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    Chapter 6 Isolation of Plant Nuclei at Defined Cell Cycle Stages Using EdU Labeling and Flow Cytometry.
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    Chapter 7 Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes.
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    Chapter 8 Fluorescence In Situ Hybridization on Rice Chromosomes.
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    Chapter 9 Dynamics of the Plant Nuclear Envelope During Cell Division
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    Chapter 10 Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana.
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    Chapter 11 In Vivo Imaging of Microtubule Organization in Dividing Giant Cell.
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    Chapter 12 Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem
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    Chapter 13 Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis
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    Chapter 14 Studying Cell Division Plane Positioning in Early-Stage Embryos
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    Chapter 15 Characterization of Cytokinetic Mutants Using Small Fluorescent Probes
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    Chapter 16 Using Live-Cell Markers in Maize to Analyze Cell Division Orientation and Timing.
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    Chapter 17 Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe.
Attention for Chapter 2: Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells
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Chapter title
Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells
Chapter number 2
Book title
Plant Cell Division
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3142-2_2
Pubmed ID
26659951
Book ISBNs
978-1-4939-3141-5, 978-1-4939-3142-2
Authors

Henrik Buschmann

Abstract

The continuing analysis of plant cell division will require additional protein localization studies. This is greatly aided by GFP-technology, but plant transformation and the maintenance of transgenic lines can present a significant technical bottleneck. In this chapter I describe a method for the Agrobacterium-mediated genetic transformation of tobacco BY-2 cells. The method allows for the microscopic analysis of fluorescence-tagged proteins in dividing cells in within 2 days after starting a coculture. This transient transformation procedure requires only standard laboratory equipment. It is hoped that this rapid method would aid researchers conducting live-cell localization studies in plant mitosis and cytokinesis.

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Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 41%
Student > Bachelor 2 12%
Researcher 2 12%
Student > Ph. D. Student 1 6%
Student > Postgraduate 1 6%
Other 0 0%
Unknown 4 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 47%
Agricultural and Biological Sciences 5 29%
Chemical Engineering 1 6%
Unknown 3 18%

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