Chapter title |
An orthogonal fusion tag for efficient protein purification.
|
---|---|
Chapter number | 18 |
Book title |
Protein Downstream Processing
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-62703-977-2_18 |
Pubmed ID | |
Book ISBNs |
978-1-62703-976-5, 978-1-62703-977-2
|
Authors |
Nilvebrant J, Astrand M, Hober S, Johan Nilvebrant, Mikael Åstrand, Sophia Hober, Nilvebrant, Johan, Åstrand, Mikael, Hober, Sophia |
Abstract |
Protein fusion tags are important tools in research when robust methods for protein purification and detection are required. In this chapter we present an efficient method for stringent protein purification. A small domain, denoted ABDz1, with affinity for both human serum albumin and Protein A has been developed. The purification tag is based on an albumin-binding domain from Streptococcal Protein G that was engineered to bind Protein A. The ABDz1-tag can be fused to any protein of choice and the purification can be performed using standard laboratory equipment. In this chapter a method for purification of ABDz1-tagged proteins using two successive affinity purification steps is described. |
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