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Germline Development

Overview of attention for book
Cover of 'Germline Development'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies.
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    Chapter 2 Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis
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    Chapter 3 Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter Mice
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    Chapter 4 Isolation of Human Male Germ-Line Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell Sorting
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    Chapter 5 Isolation and Purification of Murine Male Germ Cells
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    Chapter 6 Preparation of Enriched Mouse Syncitia-Free Pachytene Spermatocyte Cell Suspensions
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    Chapter 7 Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
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    Chapter 8 Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
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    Chapter 9 Identification of Novel Long Noncoding RNA Transcripts in Male Germ Cells
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    Chapter 10 Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
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    Chapter 11 Spermatogenesis in Cryptorchidism
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    Chapter 12 In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development
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    Chapter 13 Microspread Ovarian Cell Preparations for the Analysis of Meiotic Prophase Progression in Oocytes with Improved Recovery by Cytospin Centrifugation
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    Chapter 14 In Vitro Maturation (IVM) of Porcine Oocytes
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    Chapter 15 Experimental approaches to the study of human primordial germ cells.
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    Chapter 16 Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and Immunohistochemistry
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    Chapter 17 DNA methylation analysis of germ cells by using bisulfite-based sequencing methods.
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    Chapter 18 Gene Expression in Mouse Oocytes by RNA-Seq.
Attention for Chapter 1: Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies.
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Chapter title
Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies.
Chapter number 1
Book title
Germline Development
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-436-0_1
Pubmed ID
22144232
Book ISBNs
978-1-61779-435-3, 978-1-61779-436-0
Authors

Yunmin Li, Teruko Taketo, Yun-Fai Chris Lau, Li, Yunmin, Taketo, Teruko, Lau, Yun-Fai Chris

Abstract

Gonadal sex differentiation is an important developmental process, in which a bipotential primordial gonad undergoes two distinct pathways, i.e., testicular and ovarian differentiation, dependent on its genetic sex. Techniques of isolating fetal gonads at various developmental stages are valuable for studies on the molecular events involved in cell-fate determination, sex-specific somatic and germ-cell differentiation and structural organization. Here we describe various procedures for isolation of embryonic gonads at different developmental stages from embryos of timed-pregnant mice. The isolated fetal gonads can be used for a variety of studies, such as organ culture, gene and protein expression. As examples of applications, we describe the immunofluorescence detection of SOX9 expression in gonadal tissue sections and microRNAs profiling/expression in fetal gonads at a critical stage for sex determination.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 31%
Researcher 7 27%
Professor > Associate Professor 3 12%
Student > Master 2 8%
Other 1 4%
Other 2 8%
Unknown 3 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 35%
Biochemistry, Genetics and Molecular Biology 7 27%
Veterinary Science and Veterinary Medicine 2 8%
Neuroscience 2 8%
Medicine and Dentistry 1 4%
Other 0 0%
Unknown 5 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2011.
All research outputs
#18,301,870
of 22,659,164 outputs
Outputs from Methods in molecular biology
#7,806
of 13,019 outputs
Outputs of similar age
#195,918
of 244,041 outputs
Outputs of similar age from Methods in molecular biology
#325
of 473 outputs
Altmetric has tracked 22,659,164 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,019 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 244,041 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 473 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.

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