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Quantitation of mRNA by Polymerase Chain Reaction

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Cover of 'Quantitation of mRNA by Polymerase Chain Reaction'

Table of Contents

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    Book Overview
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    Chapter 1 General Aspects and Chances of Nucleic Acid Quantitation by PCR
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    Chapter 2 Design of Suitable Primers and Competitor Fragments for Quantitative PCR
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    Chapter 3 Cloning of Short DNA Fragments and In Vitro Transcription to Generate RNA Standards
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    Chapter 4 Direct Non-Isotopic Sequencing of PCR Products or Standards
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    Chapter 5 Isolation of mRNA
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    Chapter 6 Synthesis of cDNA
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    Chapter 7 Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA
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    Chapter 8 Single-Tube RT-PCR
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    Chapter 9 Nonradioactive Determination of PCR-Products by Using a DIG Labeled DNA Probe (Dot Blot)
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    Chapter 10 Nonradioactive Northern Blot Hybridization with DIG-Labeled DNA Probes
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    Chapter 11 Quantitation of mRNA by the ELOSA Technique Using External Standards
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    Chapter 12 Semiquantitative Detection of Viral DNA, e.g. for CMV, by Using the DNA Enzyme Immunoassay (DEIA)
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    Chapter 13 HPLC — Analysis of Nucleic Acids
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    Chapter 14 Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCR
Attention for Chapter 2: Design of Suitable Primers and Competitor Fragments for Quantitative PCR
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Chapter title
Design of Suitable Primers and Competitor Fragments for Quantitative PCR
Chapter number 2
Book title
Quantitation of mRNA by Polymerase Chain Reaction
Published by
Springer, Berlin, Heidelberg, January 1995
DOI 10.1007/978-3-642-79712-5_2
Book ISBNs
978-3-64-279714-9, 978-3-64-279712-5
Authors

Th. Köhler

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