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Pichia Protocols

Overview of attention for book
Cover of 'Pichia Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction
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    Chapter 2 Vectors and Strains for Expression
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    Chapter 3 DNA-Mediated Transformation
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    Chapter 4 Rational Design and Optimization of Fed-Batch and Continuous Fermentations
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    Chapter 5 Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
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    Chapter 6 Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C
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    Chapter 7 Rapid Screening of Chromatography Resins for the Purification of Proteins
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    Chapter 8 Characterization of O-Linked Saccharides on Glycoproteins
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    Chapter 9 Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids
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    Chapter 10 N-Linked Glycan Characterization of Heterologous Proteins
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    Chapter 11 Heavy Labeling of Recombinant Proteins
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    Chapter 12 Selenomethionine Labeling of Recombinant Proteins
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    Chapter 13 Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains
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    Chapter 14 Classical Genetics
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    Chapter 15 Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration
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    Chapter 16 Characterization of Protein-Protein Interactions
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    Chapter 17 Localization of Proteins and Organelles Using Fluorescence Microscopy
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    Chapter 18 Fluorescence Microscopy and Thin-Section Electron Microscopy
Attention for Chapter 5: Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
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Chapter title
Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
Chapter number 5
Book title
Pichia Protocols
Published in
Methods in molecular biology, January 2007
DOI 10.1007/978-1-59745-456-8_5
Pubmed ID
17951635
Book ISBNs
978-1-58829-429-6, 978-1-59745-456-8
Authors

Inan, Mehmet, Fanders, Sarah A, Zhang, Wenhui, Hotez, Peter J, Zhan, Bin, Meagher, Michael M, Mehmet Inan, Sarah A. Fanders, Wenhui Zhang, Peter J. Hotez, Bin Zhan, Michael M. Meagher, Fanders, Sarah A., Hotez, Peter J., Meagher, Michael M.

Abstract

Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the alpha-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 21%
Researcher 5 18%
Student > Bachelor 4 14%
Student > Doctoral Student 2 7%
Student > Master 2 7%
Other 2 7%
Unknown 7 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 50%
Medicine and Dentistry 2 7%
Chemical Engineering 1 4%
Business, Management and Accounting 1 4%
Environmental Science 1 4%
Other 2 7%
Unknown 7 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2012.
All research outputs
#20,166,700
of 22,678,224 outputs
Outputs from Methods in molecular biology
#9,820
of 13,037 outputs
Outputs of similar age
#152,127
of 156,485 outputs
Outputs of similar age from Methods in molecular biology
#41
of 46 outputs
Altmetric has tracked 22,678,224 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,037 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 156,485 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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