Chapter title |
Group I Intron Ribozymes
|
---|---|
Chapter number | 6 |
Book title |
Ribozymes
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-545-9_6 |
Pubmed ID | |
Book ISBNs |
978-1-61779-544-2, 978-1-61779-545-9
|
Authors |
Henrik Nielsen, Nielsen, Henrik |
Abstract |
Group I intron ribozymes constitute one of the main classes of ribozymes and have been a particularly important model in the discovery of key concepts in RNA biology as well as in the development of new methods. Compared to other ribozyme classes, group I intron ribozymes display considerable variation both in their structure and the reactions they catalyze. The best described pathway is the splicing pathway that results in a spliced out intron and ligated exons. This is paralleled by the circularization pathway that leads to full-length circular intron and un-ligated exons. In addition, the intronic products of these pathways have the potential to integrate into targets and to form various types of circular RNA molecules. Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols for initial characterization of new group I intron ribozymes. |
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Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 2 | 15% |
Student > Master | 2 | 15% |
Student > Doctoral Student | 1 | 8% |
Unknown | 1 | 8% |
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Medicine and Dentistry | 1 | 8% |
Other | 1 | 8% |
Unknown | 1 | 8% |