Chapter title |
Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA
|
---|---|
Chapter number | 5 |
Book title |
Nucleic Acid Crystallography
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2763-0_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2762-3, 978-1-4939-2763-0
|
Authors |
Mélanie Meyer, Benoît Masquida, Meyer, Mélanie, Masquida, Benoît |
Abstract |
Polyacrylamide gel electrophoresis (PAGE) constitutes a powerful technique for the efficient purification of RNA molecules dedicated to applications that require high purity levels. PAGE allows for the fractionation of RNA obtained from cell extracts, chemical or enzymatic synthesis, or modification experiments. Native or denaturing conditions can be chosen for analytical or preparative-scale separations and the nucleotide resolution can be tuned by changing the percentage and reticulation of the gel material. In this protocol, we focus on the preparation of milligram-scale amounts of ~200 nucleotides (nt) RNA molecules that were used in subsequent crystallization experiments. |
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