| Chapter title |
Neuropeptide RNA localization in tissue sections.
|
|---|---|
| Chapter number | 4 |
| Book title |
Neuropeptides
|
| Published in |
Methods in molecular biology, January 2011
|
| DOI | 10.1007/978-1-61779-310-3_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-309-7, 978-1-61779-310-3
|
| Authors |
Marc Landry, Shérine Abdel Salam, Marie Moftah, Landry, Marc, Salam, Shérine Abdel, Moftah, Marie |
| Abstract |
In situ hybridization has become a routine technique to provide insights into RNA localization. However, different protocols exist for multiple purposes, and it is, therefore, important to clearly define specific needs to choose the most suitable one(s). For instance, in situ hybridization can target different types of RNA, including mRNA or small noncoding RNA such as micro RNA (miRNA). Detection protocols are developed for light or electron microscopy and can be combined with immunocytochemistry to study RNA coexpression with proteins or peptides. In this chapter, we present some protocols to illustrate the diversity of in situ hybridization methods. We focus on the detection of mRNA or miRNA and show that the protocols are quite similar but use dedicated probe types, namely, oligo- or riboprobes and locked nucleic-acid probes. |
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