Chapter title |
Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.
|
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Chapter number | 4 |
Book title |
Lymphoid Organogenesis
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Published in |
Current topics in microbiology and immunology, January 2000
|
DOI | 10.1007/978-3-642-57276-0_4 |
Pubmed ID | |
Book ISBNs |
978-3-64-263186-3, 978-3-64-257276-0
|
Authors |
Just, U, Boettiger, D, Kan, O, Dexter, T M, Spooncer, E, U. Just, D. Boettiger, O. Kan, T. M. Dexter, E. Spooncer, Just, U., Boettiger, D., Kan, O., Dexter, T. M., Spooncer, E. |
Abstract |
The genes controlling self renewal in the haemopoietic system are still unknown. Using retroviral insertional mutagenesis we have established multipotent haemopoietic stem cell lines (FDCP-mix) that possess an increased self renewal capacity in vitro. To identify genes involved in the regulation of self renewal, proviral integration sites were cloned from FDCP-mix cells and used as probes to screen independently isolated FDCP-mix cell lines for a common proviral insertion site. So far, two common integration sites have been identified, A25 and M4. A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10%. Genes located at or near these sites are likely candidates for the control of self renewal of haemopoietic stem cells. |
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