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The Plant Cell Wall

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Cover of 'The Plant Cell Wall'

Table of Contents

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    Book Overview
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    Chapter 1 Plant Tissue Cultures
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    Chapter 2 Computerized Molecular Modeling of Carbohydrates
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    Chapter 3 Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS
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    Chapter 4 High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors
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    Chapter 5 Carbohydrate Gel Electrophoresis
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    Chapter 6 Capillary Electrophoresis with Detection by Laser-Induced Fluorescence
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    Chapter 7 Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls
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    Chapter 8 Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays
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    Chapter 9 Electron Tomography and Immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls
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    Chapter 10 Analysing Cellulose Biosynthesis with Confocal Microscopy
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    Chapter 11 Visual Mapping of Cell Wall Biosynthesis
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    Chapter 12 Atomic Force Microscopy of Plant Cell Walls
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    Chapter 13 Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls
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    Chapter 14 The Plant Cell Wall
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    Chapter 15 Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
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    Chapter 16 The Plant Cell Wall
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    Chapter 17 Extraction and Detection of Arabinogalactan Proteins
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    Chapter 18 Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens
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    Chapter 19 Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens
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    Chapter 20 Measuring In Vitro Extensibility of Growing Plant Cell Walls
Attention for Chapter 4: High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors
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Chapter title
High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors
Chapter number 4
Book title
The Plant Cell Wall
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-008-9_4
Pubmed ID
Book ISBNs
978-1-61779-007-2, 978-1-61779-008-9
Authors

Stephen C. Fry

Abstract

HVPE is an excellent and often overlooked method for obtaining objective and meaningful information about cell-wall "building blocks" and their metabolic precursors. It provides not only a means of analysis of known compounds but also an insight into the charge and/or mass of any unfamiliar compounds that may be encountered. It can be used preparatively or analytically. It can achieve either "class separations" (e.g. delivering all hexose monophosphates into a single pool) or the resolution of different compounds within a given class (e.g. ADP-Glc from UDP-Glc; or GlcA from GalA). All information from HVPE about charge and mass can be obtained on minute traces of analytes, especially those that have been radiolabelled, e.g. by in-vivo feeding of a (3)H- or (14)C-labelled precursor. HVPE does not usually damage the substance under investigation (unless staining is used), so samples of interest can be eluted intact from the paper ready for further analysis. Although HVPE is a technique that has been available for several decades, recently it has tended to be sidelined, possibly because the apparatus is not widely available. Interested scientists are invited to contact the author about the possibility of accessing the Edinburgh apparatus.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 29%
Professor 2 12%
Researcher 2 12%
Student > Master 1 6%
Student > Bachelor 1 6%
Other 2 12%
Unknown 4 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 41%
Biochemistry, Genetics and Molecular Biology 5 29%
Engineering 1 6%
Unknown 4 24%