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Cytochrome P450 Protocols

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Cover of 'Cytochrome P450 Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Bioluminescent Assays for Cytochrome P450 Enzymes
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    Chapter 2 Simultaneous Determination of Multiple CYP Inhibition Constants using a Cocktail-Probe Approach
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    Chapter 3 High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening
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    Chapter 4 The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
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    Chapter 5 High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators
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    Chapter 6 Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches
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    Chapter 7 Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium tuberculosis.
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    Chapter 8 An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins
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    Chapter 9 Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group
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    Chapter 10 Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes
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    Chapter 11 Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles
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    Chapter 12 Fluorescence-Based Screening of Cytochrome P450 Activities in Intact Cells
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    Chapter 13 Screening for Cytochrome P450 Reactivity with a Reporter Enzyme
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    Chapter 14 High-Throughput Fluorescence Assay of Cytochrome P450 3A4.
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    Chapter 15 Targeted Protein Capture for Analysis of Electrophile-Protein Adducts
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    Chapter 16 DNA Shuffling of Cytochrome P450 Enzymes
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    Chapter 17 Measurement of P450 Difference Spectra Using Intact Cells
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    Chapter 18 DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production
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    Chapter 19 P450 Oxidoreductase: Genotyping, Expression, Purification of Recombinant Protein, and Activity Assessments of Wild-Type and Mutant Protein
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    Chapter 20 LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450
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    Chapter 21 Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-Allele) Nomenclature Database
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    Chapter 22 Simultaneous In Vivo Phenotyping of CYP Enzymes
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    Chapter 23 Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase I Footprinting
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    Chapter 24 Isolation of Mouse Hepatocytes
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    Chapter 25 Highly Efficient SiRNA and Gene Transfer into Hepatocyte-Like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies
Attention for Chapter 24: Isolation of Mouse Hepatocytes
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Chapter title
Isolation of Mouse Hepatocytes
Chapter number 24
Book title
Cytochrome P450 Protocols
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-321-3_24
Pubmed ID
Book ISBNs
978-1-62703-320-6, 978-1-62703-321-3
Authors

Mina Edwards, Lyndsey Houseman, Ian R. Phillips, Elizabeth A. Shephard, Edwards, Mina, Houseman, Lyndsey, Phillips, Ian R., Shephard, Elizabeth A.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 22%
Student > Ph. D. Student 8 18%
Student > Master 5 11%
Student > Doctoral Student 4 9%
Other 3 7%
Other 8 18%
Unknown 7 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 27%
Medicine and Dentistry 6 13%
Pharmacology, Toxicology and Pharmaceutical Science 6 13%
Agricultural and Biological Sciences 5 11%
Business, Management and Accounting 1 2%
Other 6 13%
Unknown 9 20%