Chapter title |
VP4 and VP7 typing using monoclonal antibodies.
|
---|---|
Chapter number | 13 |
Book title |
Viral Gastroenteritis
|
Published in |
Archives of virology Supplementum, January 1996
|
DOI | 10.1007/978-3-7091-6553-9_13 |
Pubmed ID | |
Book ISBNs |
978-3-21-182875-5, 978-3-70-916553-9
|
Authors |
Coulson, B S, B. S. Coulson, Coulson, B. S. |
Abstract |
Both rotavirus outer capsid proteins, VP4 and VP7, elicit neutralizing antibodies. Neutralizing mouse monoclonal antibodies (N-MAbs) to VP7 are easily derived and have been used widely and successfully to serotype both stool-derived and culture-adapted rotaviruses by enzyme immunoassay (EIA). Generally, approximately 70% of rotaviruses in stool samples are typable by VP7 EIA, an inexpensive and practical method. Variations in antigenic regions between strains within human rotavirus serotypes 1, 2, 4, and 9 have been recorded. These have been termed monotypes because they are detected with N-MAbs. The molecular basis for monotypes has been determined by mapping mutations selected in N-MAb-resistant antigenic variants, and by sequence analysis of the gene encoding VP7 in newly recognized monotypes. Antigenic regions A, B and C in VP7 are involved. In order to detect all members of a particular VP7 serotype, it is necessary to type with a panel of N-MAbs specific for that serotype. N-MAbs to VP4 of human rotavirus are difficult to raise and few have proven suitable for VP4 serotyping by EIA. The specificity of the assay for each P type is highest when the VP7 serotype specificity of the capture antiserum is matched to the G type of the rotavirus in the test sample. The VP4 EIA gives similar typing rates to the VP7 typing EIA. N-MAbs directed to VP8, the smaller subunit of VP4 generated by proteolytic cleavage, are more likely to show serotype specificity. Some N-MAbs that select mutations in the putative fusion region of VP5, the larger subunit of VP4, show cross-reactivity with extracts of normal, uninfected MA 104 cells and with fetal bovine serum. These N-MAbs also give elevated EIA OD readings with rotavirus-positive, but previously non-reactive fecal samples which have been frozen and thawed repeatedly. Overall, VP8-reactive N-MAbs appear most suitable for VP4 typing by EIA. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 23% |
Student > Bachelor | 2 | 15% |
Researcher | 2 | 15% |
Student > Ph. D. Student | 2 | 15% |
Professor | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Medicine and Dentistry | 2 | 15% |
Immunology and Microbiology | 2 | 15% |
Agricultural and Biological Sciences | 2 | 15% |
Veterinary Science and Veterinary Medicine | 1 | 8% |
Other | 2 | 15% |
Unknown | 2 | 15% |