| Chapter title |
Lipid Membrane Encapsulation of a 3D DNA Nano Octahedron
|
|---|---|
| Chapter number | 12 |
| Book title |
3D DNA Nanostructure
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6454-3_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6452-9, 978-1-4939-6454-3
|
| Authors |
Steven D. Perrault, William M. Shih, Perrault, Steven D., Shih, William M. |
| Abstract |
Structural DNA nanotechnology methods such as DNA origami allow for the synthesis of highly precise nanometer-scale materials (Rothemund, Nature 440:297-302, 2006; Douglas et al., Nature 459:414-418, 2009). These offer compelling advantages for biomedical applications. Such materials can suffer from structural instability in biological environments due to denaturation and nuclease digestion (Hahn et al., ACS Nano 2014; Perrault and Shih, ACS Nano 8:5132-5140, 2014). Encapsulation of DNA nanostructures in a lipid membrane compartmentalizes them from their environment and prevents denaturation and nuclease digestion (Perrault and Shih, ACS Nano 8:5132-5140, 2014). Here, we describe the encapsulation of a 50 nm DNA nanostructure having the geometry of a wireframe octahedron in a phospholipid membrane containing poly-(ethylene glycol), resulting in biocompatible DNA nanostructures. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 17% |
| Student > Ph. D. Student | 2 | 17% |
| Professor | 1 | 8% |
| Student > Master | 1 | 8% |
| Researcher | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 5 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Immunology and Microbiology | 1 | 8% |
| Physics and Astronomy | 1 | 8% |
| Engineering | 1 | 8% |
| Unknown | 6 | 50% |