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Neutrophil Methods and Protocols

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Cover of 'Neutrophil Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The Role of Neutrophils in the Immune System: An Overview
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    Chapter 2 Isolation of Human Neutrophils from Venous Blood
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    Chapter 3 Neutrophil Isolation from Nonhuman Species
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    Chapter 4 Collection of In Vivo Transmigrated Neutrophils from Human Skin
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    Chapter 5 Subcellular fractionation of human neutrophils and analysis of subcellular markers.
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    Chapter 6 Neutrophil Methods and Protocols
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    Chapter 7 Measurement of Phospholipid Metabolism in Intact Neutrophils
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    Chapter 8 Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils
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    Chapter 9 Analysis of Electrophysiological Properties and Responses of Neutrophils
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    Chapter 10 Assessment of Neutrophil Apoptosis
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    Chapter 11 Microinjection Methods for Neutrophils
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    Chapter 12 Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells
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    Chapter 13 Neutrophil Methods and Protocols
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    Chapter 14 Spinning disk confocal imaging of neutrophil migration in zebrafish.
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    Chapter 15 Detection of Bidirectional Signaling During Integrin Activation and Neutrophil Adhesion
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    Chapter 16 Immunofluorescence and confocal microscopy of neutrophils.
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    Chapter 17 Expression of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils.
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    Chapter 18 Quantitative assessment of neutrophil phagocytosis using flow cytometry.
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    Chapter 19 Analysis of neutrophil bactericidal activity.
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    Chapter 20 Induction and quantification of neutrophil extracellular traps.
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    Chapter 21 Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes
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    Chapter 22 Cell-Free NADPH Oxidase Activation Assays: “In Vitro Veritas”
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    Chapter 23 Assessment of Priming of the Human Neutrophil Respiratory Burst
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    Chapter 24 Affinity purification and reconstitution of human phagocyte flavocytochrome B for detection of conformational dynamics in the membrane.
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    Chapter 25 Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies
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    Chapter 26 Genome-Scale Transcript Analyses with Human Neutrophils
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    Chapter 27 Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils
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    Chapter 28 High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis.
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    Chapter 29 Detection of intact transcription factors in human neutrophils.
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    Chapter 30 Disorders of neutrophil function: an overview.
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    Chapter 31 Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
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    Chapter 32 Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency
Attention for Chapter 20: Induction and quantification of neutrophil extracellular traps.
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Chapter title
Induction and quantification of neutrophil extracellular traps.
Chapter number 20
Book title
Neutrophil Methods and Protocols
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-845-4_20
Pubmed ID
Book ISBNs
978-1-62703-844-7, 978-1-62703-845-4
Authors

Gonzalez AS, Bardoel BW, Harbort CJ, Zychlinsky A, Alejandro Sanchez Gonzalez, Bart W. Bardoel, Christopher J. Harbort, Arturo Zychlinsky, Gonzalez, Alejandro Sanchez, Bardoel, Bart W., Harbort, Christopher J., Zychlinsky, Arturo

Abstract

Neutrophil extracellular trap (NET) formation is a recently discovered process in the field of innate immunity. It is important to have consistent standards in inducing and quantifying NET formation to compare data from different labs in this new area of investigation. Here we describe the conditions of neutrophil isolation from peripheral blood and stimulation that we find allow the study of NETosis in vitro. The criteria for conclusively identifying the process of NETosis, and the pros and cons of various quantification methods are discussed.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 2%
Unknown 55 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 18%
Student > Master 8 14%
Student > Bachelor 7 13%
Researcher 6 11%
Student > Postgraduate 4 7%
Other 9 16%
Unknown 12 21%
Readers by discipline Count As %
Medicine and Dentistry 13 23%
Agricultural and Biological Sciences 11 20%
Biochemistry, Genetics and Molecular Biology 7 13%
Immunology and Microbiology 7 13%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Other 4 7%
Unknown 12 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2014.
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#20,239,689
of 22,766,595 outputs
Outputs from Methods in molecular biology
#9,865
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Outputs of similar age
#268,328
of 310,339 outputs
Outputs of similar age from Methods in molecular biology
#436
of 654 outputs
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