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Macromolecular Crystallography Protocols

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Cover of 'Macromolecular Crystallography Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag
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    Chapter 2 Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project
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    Chapter 3 Baculoviral Expression of an Integral Membrane Protein for Structural Studies
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    Chapter 4 Protein Engineering
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    Chapter 5 Macromolecular Crystallography Protocols
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    Chapter 6 How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals
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    Chapter 7 Screening and Optimization Methods for Nonautomated Crystallization Laboratories
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    Chapter 8 Improving Marginal Crystals
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    Chapter 9 Optimization Techniques for Automation and High Throughput
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    Chapter 10 Three-Dimensional Crystallization of Membrane Proteins
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    Chapter 11 Crystallization of Protein-DNA Complexes
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    Chapter 12 Preparation and Crystallization of RNA
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    Chapter 13 Crystallization of RNA-Protein Complexes
Attention for Chapter 1: A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag
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