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Mendeley readers
| Chapter title |
HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.
|
|---|---|
| Chapter number | 26 |
| Book title |
Clinical Applications of Capillary Electrophoresis
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-029-8_26 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-028-1, 978-1-62703-029-8
|
| Authors |
Ewa H. Lavant, Joyce Carlson |
| Abstract |
The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Nursing and Health Professions | 1 | 100% |