| Chapter title |
A Culture Model to Study Neuron-Schwann Cell-Astrocyte Interactions
|
|---|---|
| Chapter number | 17 |
| Book title |
Schwann Cells
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7649-2_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7648-5, 978-1-4939-7649-2
|
| Authors |
Susana R. Cerqueira, Yee-Shuan Lee, Mary Bartlett Bunge, Cerqueira, Susana R., Lee, Yee-Shuan, Bunge, Mary Bartlett |
| Abstract |
In vitro models using Schwann cell and astrocyte co-cultures have been used to understand the mechanisms underlying the formation of boundaries between these cells in vivo. Schwann cell/astrocyte co-cultures also mimic the in vivo scenario of a transplant in a spinal cord injury site, thereby allowing testing of therapeutic approaches. In this chapter, we describe a triple cell culture system with Schwann cells, astrocytes, and neurons that replicates axon growth from a Schwann cell graft into an astrocyte-rich region. In vitro studies using this model can accelerate the discovery of more effective therapeutic combinations to be used along with Schwann cell transplantation after spinal cord injuries. |
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Mendeley readers
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| Student > Bachelor | 2 | 13% |
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