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RT-PCR Protocols

Overview of attention for book
Cover of 'RT-PCR Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology
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    Chapter 2 Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer
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    Chapter 3 Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR
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    Chapter 4 Quantitative RT-PCR Methods for Mature microRNA Expression Analysis
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    Chapter 5 Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction
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    Chapter 6 Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR
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    Chapter 7 Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR
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    Chapter 8 RT-PCR Protocols
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    Chapter 9 Detection of antisense RNA transcripts by strand-specific RT-PCR.
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    Chapter 10 RT-PCR Amplification and Cloning of Large Viral Sequences
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    Chapter 11 One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification
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    Chapter 12 Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR
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    Chapter 13 Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction
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    Chapter 14 The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes.
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    Chapter 15 The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification
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    Chapter 16 Skeletal Muscle RNA Extraction in Preparation for RT-PCR
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    Chapter 17 Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay
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    Chapter 18 Primer Design for RT-PCR
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    Chapter 19 Hot Start PCR
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    Chapter 20 Real-time RT-PCR for Automated Detection of HIV-1 RNA During Blood Donor Screening
Attention for Chapter 9: Detection of antisense RNA transcripts by strand-specific RT-PCR.
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Chapter title
Detection of antisense RNA transcripts by strand-specific RT-PCR.
Chapter number 9
Book title
RT-PCR Protocols
Published in
Methods in molecular biology, March 2010
DOI 10.1007/978-1-60761-629-0_9
Pubmed ID
20300995
Book ISBNs
978-1-60761-628-3, 978-1-60761-629-0
Authors

Ho EC, Donaldson ME, Saville BJ, Eric C. H. Ho, Michael E. Donaldson, Barry J. Saville

Abstract

Comprehensive genome annotation requires extensive cDNA analysis. This analysis has identified natural antisense transcripts (NATs), which are distinct from the microRNAs, siRNAs, and piRNAs, in a number of diverse eukaryotes. This wide conservation supports the possibility of an important role for NATs in regulating cellular processes. Investigating their roles requires the confirmation of expressed sequence tag (EST) data and the detection of antisense transcripts in distinct cellular backgrounds. This chapter describes the use of a reverse transcription polymerase chain reaction (RT-PCR) method for the detection of antisense transcripts. The protocol was designed to reduce the number of first strand synthesis reactions during screening for antisense transcripts through the utilization of antisense directed primers and oligo dT to prime first strand synthesis. These results are further confirmed using sense and antisense directed primers in first strand synthesis. Results indicate that optimization of the screens requires proper controls to confirm removal of gDNA contamination and to rule out self-priming as a source of first strand products.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 1%
Brazil 1 1%
United Kingdom 1 1%
Belgium 1 1%
United States 1 1%
Unknown 79 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 25%
Student > Ph. D. Student 17 20%
Student > Bachelor 8 10%
Student > Master 8 10%
Student > Postgraduate 5 6%
Other 11 13%
Unknown 14 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 32 38%
Biochemistry, Genetics and Molecular Biology 25 30%
Medicine and Dentistry 6 7%
Neuroscience 2 2%
Immunology and Microbiology 2 2%
Other 2 2%
Unknown 15 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2012.
All research outputs
#18,309,495
of 22,669,724 outputs
Outputs from Methods in molecular biology
#7,827
of 13,037 outputs
Outputs of similar age
#85,071
of 94,174 outputs
Outputs of similar age from Methods in molecular biology
#15
of 20 outputs
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So far Altmetric has tracked 13,037 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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