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Mendeley readers
Chapter title |
Construction of Unmarked Deletion Mutants in Mycobacteria
|
---|---|
Chapter number | 19 |
Book title |
Mycobacteria Protocols
|
Published in |
Methods in molecular biology, January 2009
|
DOI | 10.1007/978-1-59745-207-6_19 |
Pubmed ID | |
Book ISBNs |
978-1-58829-889-8, 978-1-59745-207-6
|
Authors |
Houhui Song, Frank Wolschendorf, Michael Niederweis, Song, Houhui, Wolschendorf, Frank, Niederweis, Michael |
Abstract |
Site-specific recombinases such as the Saccharomyces cerevisiae Flp and the P1 phage Cre proteins have been increasingly used for the construction of unmarked deletions in bacteria. Both systems consist of an antibiotic resistance gene flanked by recognition sites in direct orientation and a curable plasmid for temporary expression of the respective recombinase gene. In this chapter, we describe strategies and methods of how to use sequence-specific recombination mediated by Flp and Cre to construct mutants of Mycobacterium smegmatis, Mycobacterium bovis BCG, and Mycobacterium tuberculosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 4% |
South Africa | 1 | 4% |
Unknown | 23 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 32% |
Student > Ph. D. Student | 6 | 24% |
Student > Master | 4 | 16% |
Student > Bachelor | 2 | 8% |
Student > Doctoral Student | 1 | 4% |
Other | 4 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 40% |
Biochemistry, Genetics and Molecular Biology | 4 | 16% |
Immunology and Microbiology | 4 | 16% |
Unspecified | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Other | 4 | 16% |
Unknown | 1 | 4% |