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DNA Replication

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Cover of 'DNA Replication'

Table of Contents

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    Book Overview
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    Chapter 1 A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
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    Chapter 2 Microscopy techniques to examine DNA replication in fission yeast.
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    Chapter 3 High-Resolution Analysis of Mammalian DNA Replication Units
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    Chapter 4 Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing
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    Chapter 5 Measuring DNA content by flow cytometry in fission yeast.
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    Chapter 6 Incorporation of thymidine analogs for studying replication kinetics in fission yeast.
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    Chapter 7 EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.
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    Chapter 8 Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
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    Chapter 9 Measuring ribonucleotide incorporation into DNA in vitro and in vivo.
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    Chapter 10 Detection and Sequencing of Okazaki Fragments in S. cerevisiae.
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    Chapter 11 ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin.
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    Chapter 12 Chromatin immunoprecipitation to detect DNA replication and repair factors.
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    Chapter 13 Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism.
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    Chapter 14 Single-Molecule Observation of Prokaryotic DNA Replication
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    Chapter 15 Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.
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    Chapter 16 Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
  18. Altmetric Badge
    Chapter 17 Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes
Attention for Chapter 8: Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
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Chapter title
Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
Chapter number 8
Book title
DNA Replication
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2596-4_8
Pubmed ID
Book ISBNs
978-1-4939-2595-7, 978-1-4939-2596-4
Authors

Jia, Shaodong, Marjavaara, Lisette, Buckland, Robert, Sharma, Sushma, Chabes, Andrei, Shaodong Jia, Lisette Marjavaara, Robert Buckland, Sushma Sharma, Andrei Chabes

Abstract

DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 36%
Student > Doctoral Student 3 21%
Student > Ph. D. Student 2 14%
Student > Master 2 14%
Professor > Associate Professor 1 7%
Other 0 0%
Unknown 1 7%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 43%
Agricultural and Biological Sciences 4 29%
Chemistry 2 14%
Unknown 2 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2015.
All research outputs
#20,273,512
of 22,805,349 outputs
Outputs from Methods in molecular biology
#9,905
of 13,120 outputs
Outputs of similar age
#295,802
of 353,075 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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