| Chapter title |
Chemotaxis
|
|---|---|
| Chapter number | 2 |
| Book title |
Chemotaxis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3480-5_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3478-2, 978-1-4939-3480-5
|
| Authors |
McClure, Allison W, Wu, Chi-Fang, Johnson, Sam A, Lew, Daniel J, McClure, Allison W., Johnson, Sam A., Lew, Daniel J., Allison W. McClure, Chi-Fang Wu, Sam A. Johnson, Daniel J. Lew |
| Abstract |
We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected aspects of polarization including transient intermediates with more than one polarity cluster, oscillatory clustering of polarity factors, and mobile "wandering" polarity sites. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 27% |
| Professor | 1 | 9% |
| Student > Ph. D. Student | 1 | 9% |
| Researcher | 1 | 9% |
| Unknown | 5 | 45% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Environmental Science | 1 | 9% |
| Agricultural and Biological Sciences | 1 | 9% |
| Physics and Astronomy | 1 | 9% |
| Unknown | 6 | 55% |