| Chapter title |
A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth
|
|---|---|
| Chapter number | 15 |
| Book title |
Adult Stem Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6756-8_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6754-4, 978-1-4939-6756-8
|
| Authors |
Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, Simon D. Tran, Bakkar, Mohammed, Liu, Younan, Fang, Dongdong, Stegen, Camille, Su, Xinyun, Ramamoorthi, Murali, Lin, Li-Chieh, Kawasaki, Takako, Makhoul, Nicholas, Pham, Huan, Sumita, Yoshinori, Tran, Simon D. |
| Abstract |
This chapter describes a simplified method that allows the systematic isolation of multiple types of dental stem cells such as dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC), and stem cells of the apical papilla (SCAP) from a single tooth. Of specific interest is the modified laboratory approach to harvest/retrieve the dental pulp tissue by minimizing trauma to DPSC by continuous irrigation, reduction of frictional heat from the bur rotation, and reduction of the bur contact time with the dentin. Also, the use of a chisel and a mallet will maximize the number of live DPSC for culture. Steps demonstrating the potential for multiple cell differentiation lineages of each type of dental stem cell into either osteocytes, adipocytes, or chondrocytes are described. Flow cytometry, with a detailed strategy for cell gating and analysis, is described to verify characteristic markers of human mesenchymal multipotent stromal cells (MSC) from DPSC, PDLSC, or SCAP for subsequent experiments in cell therapy and in tissue engineering. Overall, this method can be adapted to any laboratory with a general setup for cell culture experiments. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 3% |
| Unknown | 38 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 15% |
| Student > Bachelor | 5 | 13% |
| Student > Master | 4 | 10% |
| Professor | 3 | 8% |
| Researcher | 2 | 5% |
| Other | 8 | 21% |
| Unknown | 11 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 14 | 36% |
| Biochemistry, Genetics and Molecular Biology | 4 | 10% |
| Agricultural and Biological Sciences | 2 | 5% |
| Engineering | 2 | 5% |
| Neuroscience | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 13 | 33% |