| Chapter title |
Luciferase Genes as Reporter Reactions: How to Use Them in Molecular Biology?
|
|---|---|
| Chapter number | 325 |
| Book title |
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3
|
| Published in |
Advances in biochemical engineering biotechnology, January 2015
|
| DOI | 10.1007/10_2015_325 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-927405-8, 978-3-31-927407-2
|
| Authors |
L. Cevenini, M. M. Calabretta, D. Calabria, A. Roda, E. Michelini, Cevenini, L., Calabretta, M. M., Calabria, D., Roda, A., Michelini, E. |
| Abstract |
: The latest advances in molecular biology have made available several biotechnological tools that take advantage of the high detectability and quantum efficiency of bioluminescence (BL), with an ever-increasing number of novel applications in environmental, pharmaceutical, food, and forensic fields. Indeed, BL proteins are being used to develop ultrasensitive binding assays and cell-based assays, thanks to their high detectability and to the availability of highly sensitive BL instruments. The appealing aspect of molecular biology tools relying on BL reactions is their general applicability in both in vitro assays, such as cell cultures or purified proteins, and in vivo settings, such as in whole-animal BL imaging. The aim of this chapter is to provide the reader with an overview of state-of-the-art bioluminescent tools based on luciferase genes, highlighting molecular biology strategies that have been applied so far, together with some selected examples. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 3% |
| Unknown | 36 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 22% |
| Researcher | 5 | 14% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Master | 2 | 5% |
| Student > Doctoral Student | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 13 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 32% |
| Agricultural and Biological Sciences | 3 | 8% |
| Medicine and Dentistry | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Unspecified | 1 | 3% |
| Other | 5 | 14% |
| Unknown | 13 | 35% |