| Chapter title |
Detecting p53 Isoforms at Protein Level.
|
|---|---|
| Chapter number | 2 |
| Book title |
p53 Protocols
|
| Published in |
Methods in molecular biology, November 2012
|
| DOI | 10.1007/978-1-62703-236-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-235-3, 978-1-62703-236-0
|
| Authors |
Marcel V, Khoury MP, Fernandes K, Diot A, Lane DP, Bourdon JC, Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane, Jean-Christophe Bourdon, Marcel, Virginie, Khoury, Marie P., Fernandes, Kenneth, Diot, Alexandra, Lane, David P., Bourdon, Jean-Christophe |
| Abstract |
The human p53 protein isoforms are expressed in several cell lines and modulate p53 tumor suppressor -activity, mainly through modulation of gene expression (1-4). Thus, identifying the pattern of p53 isoforms expression in cell lines is a key step for future studies of the p53 network (5). At the moment, the detection of p53 protein isoforms is based on the use of a panel of antibodies allowing their identification by comparing their molecular weights and their detection pattern by different antibodies (6). Here, classical protocols supplemented with technical know-how are described to detect p53 protein isoforms at protein level by Western blotting and immunoprecipitation. Furthermore, a simple method to study the impact of p53 protein isoforms on p53 transcriptional activity through luciferase reporter gene assays is provided. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 21% |
| Researcher | 7 | 18% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Master | 6 | 15% |
| Professor > Associate Professor | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 6 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 33% |
| Biochemistry, Genetics and Molecular Biology | 12 | 31% |
| Immunology and Microbiology | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Earth and Planetary Sciences | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 8 | 21% |