↓ Skip to main content

Legume Genomics

Overview of attention for book
Cover of 'Legume Genomics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Model Legume Genomes
  3. Altmetric Badge
    Chapter 2 Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization
  4. Altmetric Badge
    Chapter 3 Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
  5. Altmetric Badge
    Chapter 4 RNA-Seq for Transcriptome Analysis in Non-model Plants.
  6. Altmetric Badge
    Chapter 5 Functional analysis of legume genome arrays.
  7. Altmetric Badge
    Chapter 6 Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
  8. Altmetric Badge
    Chapter 7 Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
  9. Altmetric Badge
    Chapter 8 Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines.
  10. Altmetric Badge
    Chapter 9 Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection
  11. Altmetric Badge
    Chapter 10 High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants
  12. Altmetric Badge
    Chapter 11 Legume Genomics
  13. Altmetric Badge
    Chapter 12 Gene Silencing in Medicago truncatula Roots Using RNAi.
  14. Altmetric Badge
    Chapter 13 Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin ( Lupinus angustifolius )
  15. Altmetric Badge
    Chapter 14 Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens
  16. Altmetric Badge
    Chapter 15 Legume Genomics
  17. Altmetric Badge
    Chapter 16 Subcellular Localization of Transiently Expressed Fluorescent Fusion Proteins
  18. Altmetric Badge
    Chapter 17 Proteomics and the Analysis of Nodulation
  19. Altmetric Badge
    Chapter 18 Phosphoproteomic Analysis of Peptides
  20. Altmetric Badge
    Chapter 19 Plant Metabolomics: From Experimental Design to Knowledge Extraction
Attention for Chapter 12: Gene Silencing in Medicago truncatula Roots Using RNAi.
Altmetric Badge

Mentioned by

twitter
1 X user

Readers on

mendeley
35 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Gene Silencing in Medicago truncatula Roots Using RNAi.
Chapter number 12
Book title
Legume Genomics
Published in
Methods in molecular biology, September 2013
DOI 10.1007/978-1-62703-613-9_12
Pubmed ID
Book ISBNs
978-1-62703-612-2, 978-1-62703-613-9
Authors

Floss DS, Schmitz AM, Starker CG, Gantt JS, Harrison MJ, Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, Maria J. Harrison, Floss, Daniela S., Schmitz, Alexa M., Starker, Colby G., Gantt, J. Stephen, Harrison, Maria J.

Abstract

Medicago truncatula is used widely as a model system for studies of root symbioses, interactions with parasitic nematodes and fungal pathogens, as well as studies of development and secondary metabolism. In Medicago truncatula as well as other legumes, RNA interference (RNAi) coupled with Agrobacterium rhizogenes-mediated root transformation, has been used very successfully for analyses of gene function in roots. One of the major advantages of this approach is the ease and relative speed with which transgenic roots can be generated. There are several methods, both for the generation of the RNAi constructs and the root transformation. Here we provide details of an RNAi and root transformation protocol that has been used successfully in M. truncatula and which can be scaled up to enable the analysis of several hundred constructs.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 3%
United States 1 3%
Unknown 33 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 26%
Researcher 7 20%
Student > Master 6 17%
Student > Bachelor 4 11%
Other 3 9%
Other 3 9%
Unknown 3 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 54%
Biochemistry, Genetics and Molecular Biology 6 17%
Computer Science 2 6%
Unspecified 1 3%
Veterinary Science and Veterinary Medicine 1 3%
Other 2 6%
Unknown 4 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 March 2014.
All research outputs
#20,226,756
of 22,751,628 outputs
Outputs from Methods in molecular biology
#9,859
of 13,088 outputs
Outputs of similar age
#172,390
of 196,918 outputs
Outputs of similar age from Methods in molecular biology
#45
of 60 outputs
Altmetric has tracked 22,751,628 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 196,918 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.