| Chapter title |
Applications of mouse airway epithelial cell culture for asthma research.
|
|---|---|
| Chapter number | 7 |
| Book title |
Mouse Models of Allergic Disease
|
| Published in |
Methods in molecular biology, August 2013
|
| DOI | 10.1007/978-1-62703-496-8_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-495-1, 978-1-62703-496-8
|
| Authors |
Horani A, Dickinson JD, Brody SL, Amjad Horani, John D. Dickinson, Steven L. Brody, Horani, Amjad, Dickinson, John D., Brody, Steven L. |
| Abstract |
Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell-cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal epithelial cell cultures for the study of asthma biology. A major advantage of this system is the ability to use airway epithelial cells from mice with defined genetic backgrounds. The in vitro proliferation and differentiation of mouse airway epithelial cells uses the air-liquid interface condition to generate well-differentiated epithelia with characteristics of native airways. Protocols are provided for manipulation of differentiation, induction of mucous cell metaplasia, genetic modification, and cell and pathogen coculture. Assays for the assessment of gene expression, responses of cells, and analysis of specific cell subpopulations within the airway epithelium are included. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 30% |
| Student > Ph. D. Student | 5 | 25% |
| Student > Doctoral Student | 2 | 10% |
| Unspecified | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 3 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 30% |
| Medicine and Dentistry | 3 | 15% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Unspecified | 1 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Other | 4 | 20% |
| Unknown | 3 | 15% |